Unstained protein ladder western blot verification#
Unstained protein ladder western blot plus#

Under suggested conditions, the CosmoPAGE WB Ladder resolves 4 prestained bands on the membrane and 10 bands after secondary antibodies binding followed by chemiluminescent detection. Recombinant IgG binding proteins, Glycerol and SDS. Apply more for thicker (> 1.5 mm) or larger gel.2.5~5 μl per well for one-step Western blot using 1st Ab conjugated with reporter enzymes.1.5~2.5 μl per well for two-step Western blot using 1st Ab followed with 2nd Ab conjugated with reporter enzymes.Compatible with chemiluminescent substrates and fluorescent secondary antibodies. Wide range size estimation (10-200 kDa) Western blot confirmationdetect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein Applications Western blotting: detection of the two unstained bands via the detection method used for the target protein.The ladder is visualized by SDS-PAGE using coomassie or silver stains or detected in Western blots with protein stains. Load directly on gelsno pre-heating is necessary. The Thermo ScientificTM PageRulerTM Unstained Protein Ladder consists of a unstained mixture of 14 recombinant, highly purified proteins ranging from 10 kDa to 200 kDa.

When used during SDS-PAGE, these protein ladder sets provide molecular weight markers ranging from 10250 kDa (unstained ladder) or 10210 kDa (pre-stained ladder).

10 IgG-binding proteins for visualization on Western blots Convenient and ready-to-use CLEARLY Protein Ladder sets are mixtures of 11 highly purified recombinant proteins.

4 prestained proteins for clear visualization during electrophoresis and Western blotting transfer.

Do NOT heat, dilute, or add reducing agents before loading. This product is supplied in gel loading buffer and is ready to use. In addition, CosmoPAGE WB Ladder has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). CosmoPAGE WB Ladder is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, the ladder proteins contain an integral Step-tag II Sequence and may be detected on Western blots using Strep-Tactin Conjugates. Second, ten IgG- binding proteins can be immuno-detected on film or by CCD imaging. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains.

Unstained protein ladder western blot verification#

First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDS- PAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. CosmoPAGE WB Ladder performs dual functions. Additional data should be considered for a more accurate adjustment.Ġ.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.ĥμl of IRIS9 Prestained Protein Ladder (PM009) resolves 9 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.CosmoPAGE WB Ladder is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in Tris- Glycine buffer. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. 2.5 μl per well for general Western transferring. Refer to the IRIS9 Prestained Protein Ladder patterns in various electrophoresis conditions:ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Guide for Molecular Weight Estimation (kDa)

Unstained protein ladder western blot plus#

Precision Plus Protein Unstained Recombinant Protein Standards are Strep-tagged, enabling immunodetection and molecular weight determination on western blots. The IRIS9 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. Unstained natural protein standards allow accurate MW determination with uniform band intensities on SDS-PAGE gels stained with Coomassie Blue or zinc. The 9 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 30 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The protein marker covers a broad molecular weight range from 10 to 200 kDa (12 bands, separation on a SDS Tris. The IRIS9 Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 180 kDa.